Isolation, sequencing and comparative analysis of Bacillus pumilus plasmids

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Doklady Bashkirskogo Universiteta. 2018. Volume 3. No. 4. pp. 397-402.

Authors

Evdokimova O. V.*
Institute of Microbiology, National Academy of Sciences
2 Kuprevich Street, 220141 Minsk, Belarus
Valentovich L. N.
Institute of Microbiology, National Academy of Sciences; Belarusian State University
2 Kuprevich Street, 220141 Minsk, Belarus; 4 Niezaliežnasci Avenue, 220030 Minsk, Belarus

Abstract

Plasmids of natural B. pumilus bacteria isolated in Belarus were recovered. Extrachromosomal genetic elements were divided into 7 subgroups by size and restriction profile. The complete nucleotide sequences of plasmids representing each of subgroups were determined, allowing to affiliate them to pC194 family (RCR type). Comparative analysis of nucleotide sequences and predicted amino acid sequences has revealed conservative loci typical for most plasmids of the family. The unique modules (migrating element pBP-T2 and ParABC pBR-6322) were detected.

Keywords

  • Bacillus pumilus
  • extrachromosomal genetic elements
  • plasmids
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