Biochemical assessment androclinic lines of spring rape (Brassica Napus L.)

Doklady Bashkirskogo Universiteta. 2018. Volume 3. No. 4. pp. 432-438.

Authors


Muravlev A.*
All-Russian Rapeseed Research Institute
26 Boevoy proezd, 398037 Lipetsk, Russia
Chesnokova E.
All-Russian Rapeseed Research Institute
26 Boevoy proezd, 398037 Lipetsk, Russia
Shilovskih I.
All-Russian Rapeseed Research Institute
26 Boevoy proezd, 398037 Lipetsk, Russia

Abstract


The modern level of processing industry development places high demands on breeds of spring planted rape. The necessity of creating new breeds with improved fatty-acid composition has become obvious. Mutagenesis, genetic transformation and cross-species hybridization are used to create them. The possibility of using biotechnical methods in creation of new plants with required features is becoming interesting. Androclinium lines of spring rape are obtained by using anther cultures in vitro . Anthers cultures were isolated from the donor plant breed Likolli. Seeds bio-chemical analysis has shown difference in the composition of fatty acids between the lines and donor breed. Lines (AL22 and AL28), containing more than 68.0% oleic acid, were extracted which in donor breed was 64.97%. Changes were also found with regard to composition of palmitic (C16:0), linoleic (C18:2), linolenic (C18:3) and eicosenic (C20:l) acids.

Keywords


  • линоленовая кислота
  • rape
  • anther
  • androclinium lines
  • fatty-acid composition oleic acid
  • linoleic acid
  • linolenic acid