Determining of the activity of amylolytic enzymes using substrate immobilized in polyacrylamide gel

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Doklady Bashkirskogo Universiteta. 2017. Volume 2. No. 5. pp. 736-740.

Authors

Maksutova V. O.*
Bashkir State University
32 Zaki Validi Street, 450076 Ufa, Republic of Bashkortostan, Russia
Tsvetkov V. O.
Bashkir State University
32 Zaki Validi Street, 450076 Ufa, Republic of Bashkortostan, Russia
Shpirnaya I. A.
Bashkir State University
32 Zaki Validi Street, 450076 Ufa, Republic of Bashkortostan, Russia
Ibragimov R. I.
Bashkir State University
32 Zaki Validi Street, 450076 Ufa, Republic of Bashkortostan, Russia

Abstract

In the work, the amylolytic activity of amylase preparations was determined using a substrate immobilized in a polyacrylamide gel. The preparation used amylases of Bacillus subtilis, Leptinotarsa decemlineata Say and human saliva. Also, the experiments were conducted to determine the effect of the pH of the solution and the concentration of the salt on the gel. The images of the gels obtained after staining were analyzed using a computer program to determine the average color value of individual areas in the image.

Keywords

  • amylolytic enzymes
  • activity determination
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