PCR-analysis of the monogizigenase coding tbmD gene in bacteria - destructors of chlororomatic derivatives
Doklady Bashkirskogo Universiteta. 2018. Volume 3. No. 6. pp. 626-630.
Authors
Starikov S. N.*
Ufa Institute of Biology UFRC RAS
69 Prospect Octobrya Street, 450054 Ufa, Republic of Bashkortostan, Russia
*E-mail: senik0406@gmail.com
Sagitova A. I.
Ufa Institute of Biology UFRC RAS; Educational and Scientific Center BSPU named after M. Akmulla and UIB UFRC RAS
69 Prospect Octobrya Street, 450054 Ufa, Republic of Bashkortostan, Russia; 3-a Oktyabr’skoy Revolyutsii Street, 450008 Ufa, Republic of Bashkortostan, Russia
Yakshidavletova L. I.
Bashkir State University
32 Zaki Validi Street, 450074 Ufa, Republic of Bashkortostan, Russia
Chizhkova A. P.
Ufa Institute of Biology UFRC RAS; Bashkir State Pedagogical University named after M. Akmulla
69 Prospect Octobrya Street, 450054 Ufa, Republic of Bashkortostan, Russia; 3-a Oktyabr’skoy Revolyutsii Street, 450008 Ufa, Republic of Bashkortostan, Russia
Galyautdinova Y. A.
Ufa Institute of Biology UFRC RAS; Bashkir State Pedagogical University named after M. Akmulla
69 Prospect Octobrya Street, 450054 Ufa, Republic of Bashkortostan, Russia; 3-a Oktyabr’skoy Revolyutsii Street, 450008 Ufa, Republic of Bashkortostan, Russia
Markusheva T. V.
Ufa Institute of Biology UFRC RAS; Bashkir State Pedagogical University named after M. Akmulla; Educational and Scientific Center BSPU named after M. Akmulla and UIB UFRC RAS
69 Prospect Octobrya Street, 450054 Ufa, Republic of Bashkortostan, Russia; 3-a Oktyabr’skoy Revolyutsii Street, 450008 Ufa, Republic of Bashkortostan, Russia; 3-a Oktyabr’skoy Revolyutsii Street, 450008 Ufa, Republic of Bashkortostan, Russia
Abstract
In this study we present the data of a comparative analysis of strains-destructors of chloroaromatic compounds isolated from a population of soil microorganisms exposed to the long-term effects of petrochemical production in the Republic of Bashkortostan. PCR analysis of the destructors was performed using primers proposed by B. Hendrickx et al. to detect the conserved regions of the tbmD gene. It has been established that in the genome of Achromobacter sp. 36P, there is a similar gene encoding mono-oxygenase tbmD , while its homologs are absent in representatives of the genera Agromyces, Cellulosimicrobium, Gluconobacter, Pseudomonas and Rhodococcus . The results of the work reveal the genetic diversity of bacteria and expand the possibility of using microorganisms to maintain the quality of the environment in the technosphere.
Keywords
- bacterium
- monooxygenase
- tbmD gene
- PCR