Extraction and characterization of proteases from Phytophtora infestans

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Doklady Bashkirskogo Universiteta. 2017. Volume 2. No. 5. pp. 719-724.

Authors

Maksutova V. O.*
Bashkir State University
32 Zaki Validi Street, 450076 Ufa, Republic of Bashkortostan, Russia
Tsvetkov V. O.
Bashkir State University
32 Zaki Validi Street, 450076 Ufa, Republic of Bashkortostan, Russia
Shpirnaya I. A.
Bashkir State University
32 Zaki Validi Street, 450076 Ufa, Republic of Bashkortostan, Russia
Ibragimov R. I.
Bashkir State University
32 Zaki Validi Street, 450076 Ufa, Republic of Bashkortostan, Russia

Abstract

In the course of the work, a total protease preparation purified from an extract of homogenate of mycelium of Phytophthora infestans with a high degree of purification was obtained using a specific substrate. The presence of high proteolytic activity in a variety of fractions was shown. Also, experiments were conducted to determine the temperature stability of proteases. The method of electrophoresis in an immobilized substrate showed the proteolytic activity of individual molecular forms of proteases in cells and P. infestans culture fluid.

Keywords

  • Phytophthora infestans
  • affine chromatography
  • proteolytic enzymes
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